Network Engineering of Eukaryotic Cell Factories

Group leader: Mikael Rørdam Andersen 

Imagine a world, where biotechnology – like electricity today - is built into everything we do. Due to its versatile applications, biotech has the potential to become an integrated part of our technology-base. In the future, bio-products - from simple molecules to biological machines we can hardly imagine today - will be ubiquitous and something we take for granted.
In particular, we believe that in 10-15 years, biotech will enable the complete design and synthesis of tailor-made biological systems for production of designed products.

It is this future that our research group is dedicated to bringing about. We seek to provide fundamental insights, enabling technologies and essential datasets in the application-based areas of biotechnology.
Acknowledging that we are only in the first steps towards de novo design of cells, we focus our current efforts on network-based genetic engineering (network engineering): Quantitative analysis of cells using genome-scale technologies and data integration, and explore the data for a deeper understanding of cells and driving cell engineering.
Our general area of research and area of expertise is eukaryotic cell factories used for the production of proteins (enzymes and pharmaceutical proteins) and bioactives.

As model systems we focus on the industrially relevant microorganisms of the Aspergillus genus and Chinese hamster ovary (CHO) cells.  The Aspergilli are natural cell factories, and on average produce more than 300 potential products: 2-300 different secreted enzymes and 50-100 putative bioactives. CHO cells are the production system of choice for heterologous expression complex protein pharmaceuticals. 

Our primary technologies are: Genome-scale modeling of metabolism and cell machinery, comparative genomics, gene expression analysis by RNA-seq and DNA microarrays, bioreactor cultivation, glyco-proteomics, and integrative data analysis coupled with bioinformatics.

A key value for the group is collaboration and ties to the society. We have strong collaborative ties to biotech companies, international academic groups and other research groups at Department of Systems Biology and at the NNF Center for Biosustainability. Contact us, the future starts today!

 

Contact

Mikael Rørdam Andersen
Associate Professor
DTU Bioengineering
+45 45 25 26 75
http://www.bio.dtu.dk/english/research/research-groups/network-engineering-of-eukaryotic-cell-factories
29 MAY 2017