Group leader: Mogens Kilstrup
The vision of the Metabolic Signaling and Regulation (MSR) Group is to combine intracellular metabolomics with transcriptomics and proteomics into an integrated systems-biological understanding of cell physiology for improvement of cellular performance during industrial scale fermentations or for combatting harmful microbes.
The MSR group is unique, in its combination of an analytical chemist with expertise in cutting edge quantification techniques and microbiologists with expertise within fermentation, physiology, genetics, molecular biology, transcriptomics, proteomics and stress research. An integrated approach is used where all phases in culturing, quenching, sampling, analysis, and data handling are optimized simultaneously to obtain complementary data that can be correlated in mathematical models. Analysis is performed primarily at the population level, but also at the single cell level to address the heterogeneity in global signaling and regulation.
Because of their importance, the MSR group is focusing on phosphorylated and negatively charged metabolites, including the intermediates of central and nucleotide metabolism. Especially secondary messengers such as cAMP, cGMP, c-di-AMP, c-di-GMP, and (p)ppGpp are in focus, as they are extremely important intracellular signals for bacterial metabolism, and often determine industrial production rates and bacterial pathogenesis.
Experimental organisms cover industrially important gram-positive and gram-negative bacteria, yeast and fungy, such as Escherichia coli, Lactococcus lactis, Bacillus subtilis, Streptomyces coelicolor, Saccharomyces cerevisiae, and Aspergillus niger